My major research interest is total joint replacement, biomaterials
and tissue engineering.
The lateral view preoperative radiograph shows complete loss of
joint space and severe osteophyte formation on the posterior surfaces
of the femur and the tibia (A). The immediate postoperative view
shows good alignment of the knee and complete removal of all the
We describe a biomimetic mode of insoluble signaling stimulation to
provide target delivery of bone morphogenetic protein-2 (BMP-2),
with the aim of prolonging the retention of BMP-2 use in bone tissue
engineering and to enable its localized release in response to cellular
to tether BMP-2 onto a poly(lactide-co-glycolide) scaffold. Use of
PEG-tethered BMP-2 was feasible because BMP-2 retained its activity
after covalent conjugation. The PEG-tethered BMP-2 conjugate sustained
stimulation and retained its mitogenic activity, notably affecting pluripotent
stem cell proliferation and differentiation. We seeded the scaffolds with
bone marrow–derived mesenchymal stromal cells as progenitor cells to
evaluate their morphology and phenotypic expression. We also created
bilateral, fullthickness cranial defects in rabbits to investigate the osteogenic
effect of cultured mesenchymal stromal cells on bone regeneration in vivo.
Histomorphometry and histology demonstrated that the PEG-tethered
BMP-2 conjugate enhanced de novo bone formation after surgery.
Our work revealed the potential for biomimetic surface engineering by
entrapping signaling growth factor to stimulate osteogenesis. Our
technique may provide a new platform for bone-engineered stem cell therapies.
Scanning electron microscopy photomicrographs of the macroporous
poly(lactide-co-glycolide) (PLG) scaffold (A) and the surface of the